from left to right
Prof. RNDr. Ilona Hromadnikova, Ph.D.,
MSc. Katerina Kotlabova, MSc. Petra Pirkova, BSc. Lenka Dvorakova,
BSc. Marketa Ondrackova
Professor Ilona Hromadníková PhD
Head of Department
Department of Molecular Biology and Cell Pathology
Third Faculty of Medicine
Professor Hromadníková has 17 years experience in cell biology and immunology and is interested in the pathogenesis of leukaemia, transplant-related complications such as GvHD, rheumatoid arthritis and state of the art non-invasive prenatal diagnostics and screening methods based on the presence of extracellular nucleic acids in maternal circulation. She has been also investigating the mutual interactions between peripheral blood mononuclear cells and human leukaemia cells, with the aim of potential usage of NK cell immunotherapy for the treatment of relapsed leukaemia.
Professor Hromadníková has published papers in more than 60 peer-review journals and supervised 10 PhD students for over 10 years. Professor Hromadníková has had extensive national and internal collaboration. She was a partner to EC FP5 Eurobank, EC FP6 Transeurope, EC FP6 Marie Curie ITN- Transnet, EC FP6 STREP – Stemdiagnostics, EC FP6 Network of Excellence – SAFE and NATO Collaborative Research Grant. She has been awarded by several national and international awards for an outstanding research in the field of haematology, rheumatology and gynecology/obstetrics.
Research Network Team
Professor Ilona Hromadníková, leads the research concerning the role of hsp in juvenile idiopathic arthritis pathogenesis.
Professor Petr Sedlacek. Head of Transplant Unit, Department of Pediatric Hematology and Oncology, University Hospital Motol, 2nd Medical faculty, Charles University Prague.
Shuang Li (China) Early Stage Researcher
Shuang procured her Bachelor degree in Clinical Medicine and continued her study in biomedical sciences attaining her Master degree in 2007, majoring in Immunology at Peking University, Beijing with a thesis entitled, “Cancer-testis antigen (CTA) expression in Multiple Myeloma (MM) and its Effect Factor.”
Shuang has been employed as a Guest Scientist with Otto von Guericke University, Magdeburg, working in the Institute of Experimental Internal Medicine. Here she defined the role novel signalling components of NF-kappaB signalling pathway in the Helicobacter pylori infection model.
She has technical expertise in the methods and techniques of Western Blot, RNA interference, Real time PCR, transfection, molecular cloning, single cell genotyping and cell culture. She is also familiar with Immunohistochemistry (IHC), Co-Immunoprecipitation (Co-IP), Enzyme Linked Immunospot Assay (ELISPOT) and other immunochemical techniques.
She is co-author for a number of articles found within ‘Progress in Modern Biomedicine’ including, “The Effect of PD-L1 Signal on The Active and Apoptosis of Jurkat Cell” and
“ The study of T cell Lymphoma in Epstein-Barr Virus BLLF-1 gene Transgenic Mice”.
Project Title: GvL effects of NK cells against human leukaemia cell lines - in vitro and in vivo studies
1. Hromadníková I, Pirkova P, and Sedlackova L. Influence of in vitro IL-2 or IL-15 alone or in combination with Hsp 70 derived 14-mer peptide (TKD) on the expression of NK cell activatory and inhibitory receptors. Mediators of Inflammation 2013 (in press).
2. Sedlackova L, Spacek M, Holler E, Imryskova Z, Hromadníková I. He-shock protein expression in leukemia. Tumour Biol . 2011 Feb;32(1):33-44.
3. Hromadníková I, Volchenkov R, Sedlackova L, Spacek M, Kozak T. Expression of heat shock protein 70 and NKG2D ligands in acute myeloid leukemia cell lines. J Recept Signal Transduct Res . 2010 Jun;30(3):161-9.4. Hromadníková I, Sedlackova L. Analysis of cell surface and relative gene expression of heat shock protein 70 in human leukemia cell lines. Leuk Lymphoma . 2008 Mar;49(3):570-6.